Autor(es): Smith Darci R, Lee John S, Jahrling Jordan, Kulesh David A, Turell Michael J, Groebner Jennifer L, O'Guinn Monica L

Resumo: Chikungunya (CHIK) - O'nyong-nyong (ONN) are important emerging arthropod-borne diseases. Molecular diagnosis of these two viruses in mosquitoes has not been evaluated, - the effects of extraneous mosquito tissue on assay performance have not been tested. Additionally, no real-time reverse transcription-polymerase chain reaction (RT-PCR) assay exists for detecting ONN virus (ONNV) RNA. We describe the development of sensitive - specific real-time RT-PCR assays for detecting CHIK - ONN viral RNA in mosquitoes, which have application for field use. In addition, we compared three methods for primer/probe design for assay development by evaluating their sensitivity - specificity. This comparison resulted in development of virus-specific assays that could detect less than one plaque-forming unit equivalent of each of the viruses in mosquitoes. The use of these assays will aid in arthropod-borne disease surveillance - in the control of the associated diseases.

Imprenta: The American Journal of Tropical Medicine and Hygiene, v. 81, n. 4, p. 679-684, 2009

Identificador do objeto digital: 10.4269/ajtmh.2009.09-0138

Descritores: Chikungunya virus - Pathogenesis ; Chikungunya virus - RNA ; Chikungunya virus - Infectious diseases ; Chikungunya virus - Viral infections ; Chikungunya virus - Molecular methods ; Chikungunya virus - PCR detection ; Chikungunya virus - Real Time PCR ; Chikungunya virus - RT-PCR ; Chikungunya Virus - Virus ; Chikungunya virus - Molecular screening ; Chikungunya virus - Public health

Data de publicação: 2009

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