Development of a TaqMan array card for acute-febrile-illness outbreak investigation and surveillance of emerging pathogens, including Ebola virus
Autor(es): Liu Jie, Ochieng Caroline, Wiersma Steve, Ströher Ute, Towner Jonathan S, Whitmer Shannon, Nichol Stuart T, Moore Christopher C, Kersh Gilbert J, Kato Cecilia, Sexton Christopher, Petersen Jeannine, Massung Robert, Hercik Christine, Crump John A, Kibiki Gibson, Maro Athanasia, Mujaga Buliga, Gratz Jean, Jacob Shevin T, Banura Patrick, Scheld W Michael, Juma Bonventure, Onyango Clayton O, Montgomery Joel M, Houpt Eric, Fields Barry
Resumo: Acute febrile illness (AFI) is associated with substantial morbidity - mortality worldwide, yet an etiologic agent is often not identified. Convalescent-phase serology is impractical, blood culture is slow, - many pathogens are fastidious or impossible to cultivate. We developed a real-time PCR-based TaqMan array card (TAC) that can test six to eight samples within 2.5 h from sample to results - can simultaneously detect 26 AFI-associated organisms, including 15 viruses (chikungunya, Crimean-Congo hemorrhagic fever [CCHF] virus, dengue, Ebola virus, Bundibugyo virus, Sudan virus, hantaviruses [Hantaan - Seoul], hepatitis E, Marburg, Nipah virus, o'nyong-nyong virus, Rift Valley fever virus, West Nile virus, - yellow fever virus), 8 bacteria (Bartonella spp., Brucella spp., Coxiella burnetii, Leptospira spp., Rickettsia spp., Salmonella enterica - Salmonella enterica serovar Typhi, - Yersinia pestis), - 3 protozoa (Leishmania spp., Plasmodium spp., - Trypanosoma brucei). Two extrinsic controls (phocine herpesvirus 1 - bacteriophage MS2) were included to ensure extraction - amplification efficiency. Analytical validation was performed on spiked specimens for linearity, intra-assay precision, interassay precision, limit of detection, - specificity. The performance of the card on clinical specimens was evaluated with 1,050 blood samples by comparison to the individual real-time PCR assays, - the TAC exhibited an overall 88% (278/315; 95% confidence interval [CI], 84% to 92%) sensitivity - a 99% (5,261/5,326, 98% to 99%) specificity. This TaqMan array card can be used in field settings as a rapid screen for outbreak investigation or for the surveillance of pathogens, including Ebola virus.
Imprenta: Journal of Clinical Microbiology, v. 54, n. 1, p. 49-58, 2016
Identificador do objeto digital: 10.1128/JCM.02257-15
Descritores: Chikungunya virus - Cytopathology ; Chikungunya virus - Infectious diseases ; Chikungunya virus - Serology ; Chikungunya virus - Viral infections ; Chikungunya virus - PCR detection ; Chikungunya virus - Real Time PCR ; Chikungunya virus - Serology ; Chikungunya Virus - Virus ; Chikungunya virus - Chikungunya fever ; Chikungunya virus - Dengue
Data de publicação: 2016
