Autor(es): Saxena Parag, Dash Paban Kumar, Santhosh S R, Shrivastava Ambuj, Parida Manmohan, Rao Pv Lakshmana

Resumo: Dengue is emerging as a major public health concern in many parts of the world. The development of a one-step, single tube, rapid, - multiplex reverse transcription polymerase chain reaction (M-RT-PCR) for simultaneous detection - typing of dengue virus using serotype specific primers during acute phase of illness is reported. An optimal assay condition with zero background was established having no cross-reaction with closely related members of flavivirus (Japanese encephalitis, West Nile, Yellow fever) - alphavirus (Chikungunya). The feasibility of M-RT-PCR assay for clinical diagnosis was validated with 620 acute phase dengue patient sera samples of recent epidemics in India. The comparative evaluation vis a vis conventional virus isolation revealed higher sensitivity. None of the forty healthy serum samples screened in the present study revealed any amplification, thereby establishing specificity of the reported assay for dengue virus only. These findings clearly suggested that M-RT-PCR assay reported in the present study is the rapid - cost-effective method for simultaneous detection as well as typing of the dengue virus in acute phase patient serum samples. Thus, the M-RT-PCR assay developed in this study will serve as a very useful tool for rapid diagnosis - typing of dengue infections in endemic areas.

Imprenta: Virology Journal, v. 5, p. 20, 2008

Identificador do objeto digital: 10.1186/1743-422X-5-20

Descritores: Chikungunya virus - Cell ; Chikungunya virus - DNA ; Chikungunya virus - Flaviviridae ; Chikungunya virus - Pathogenesis ; Chikungunya virus - RNA ; Chikungunya virus - Viral infections ; Chikungunya virus - PCR detection ; Chikungunya virus - RT-PCR ; Chikungunya Virus - Virus ; Chikungunya virus - Chikungunya fever ; Chikungunya virus - Dengue ; Chikungunya virus - Epidemic ; Chikungunya virus - Public health

Data de publicação: 2008

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