Determination of Toll-like receptor-induced cytokine profiles in the blood and cerebrospinal fluid of Chikungunya patients
Autor(es)Kashyap Rajpal Singh, Morey Shweta, Bhullar Shradha, Baheti Neeraj, Chandak Nitin, Purohit Hemant, Taori Girdhar, Daginawala Hatim
ResumoChikungunya infection caused by Chikungunya virus (CHIKV) is an inflammatory disease affecting the joints - may also lead to neurological complications. We investigated a panel of human Toll-like receptor (TLR)-induced cytokines in Chikungunya patients with - without neurological complications. In a case-control study, a panel of 12 cytokines - chemokines, TNF-?, IFN-?, IL-1?, IL-6, IL-12, IL-17A, IL-8, monocyte chemotactic protein (MCP)-1, RANTES, interferon (IFN)-?-induced protein (IP)-10, monokine induced by IFN-? (MIG) - thymus - activation-regulated chemokine (TARC), was analysed using a conventional ELISA protocol in the serum samples of Chikungunya patients without neurological complications - in the cerebrospinal fluid (CSF) - paired serum samples of Chikungunya patients with neurological complications. The levels of 3 cytokines, IL-1?, IL-17A - IL-8, - 4 chemokines, MCP-1, RANTES, IP-10 - TARC, were raised in serum samples of Chikungunya patients without neurological complications, whereas, 4 cytokines, TNF-?, IFN-?, IL-6 - IL-8, - 4 chemokines, MCP-1, RANTES, MIG - TARC, were elevated in CSF samples of Chikungunya patients with neurological complications. Moreover, the levels of IL-6 - IL-8 cytokines were significantly elevated in the CSF compared to paired serum samples in Chikungunya patients with neurological complications. In CHIKV infection, multiple cytokines are elevated in serum - CSF. The elevation in IL-6 - IL-8 cytokines in CSF correlates with neurological involvement.
ImprentaNeuroimmunomodulation, v. 21, n. 6, p. 338-346, 2014
Identificador do Objeto Digital10.1159/000358240
DescritoresChikungunya virus - Biochemistry ; Chikungunya virus - Biosynthesis ; Chikungunya virus - Pathogenesis ; Chikungunya virus - Proteins ; Chikungunya virus - Cytokines ; Chikungunya virus - Viral infections ; Chikungunya Virus - Virus ; Chikungunya virus - Chikungunya fever ; Chikungunya virus - Immunology
Data de Publicação:2014
