Detection of blood-transmissible agents: Can screening be miniaturized?
Autor(es): Fournier-Wirth Chantal, Jaffrezic-Renault Nicole, Coste Joliette
Resumo: Transfusion safety relating to blood-transmissible agents is a major public health concern, particularly when faced with the continuing emergence of new infectious agents. These include new viruses appearing alongside other known reemerging viruses (West Nile virus, Chikungunya) as well as new strains of bacteria - parasites (Plasmodium falciparum, Trypanosoma cruzi) - finally pathologic prion protein (variant Creutzfeldt-Jakob disease). Genomic mutations of known viruses (hepatitis B virus, hepatitis C virus, human immunodeficiency virus) can also be at the origin of variants susceptible to escaping detection by diagnostic tests. New technologies that would allow the simultaneous detection of several blood-transmissible agents are now needed for the development - improvement of screening strategies. DNA microarrays have been developed for use in immunohematology laboratories for blood group genotyping. Their application in the detection of infectious agents, however, has been hindered by additional technological hurdles. For instance, the variability among - within genomes of interest complicate target amplification - multiplex analysis. Advances in biosensor technologies based on alternative detection strategies have offered new perspectives on pathogen detection; however, whether they are adaptable to diagnostic applications testing biologic fluids is under debate. Elsewhere, current nanotechnologies now offer new tools to improve the sample preparation, target capture, - detection steps. Second-generation devices combining micro- - nanotechnologies have brought us one step closer to the potential development of innovative - multiplexed approaches applicable to the screening of blood for transmissible agents.
Imprenta: Transfusion, v. 50, n. 9, p. 2032-2045, 2010
Identificador do objeto digital: 10.1111/j.1537-2995.2010.02678.x
Descritores: Chikungunya virus - DNA ; Chikungunya virus - Pathogenesis ; Chikungunya virus - Infectious diseases ; Chikungunya virus - Viral infections ; Chikungunya Virus - Virus ; Chikungunya virus - Transmission ; Chikungunya virus - Public health
Data de publicação: 2010
