Correlation of plasma viral loads and presence of Chikungunya IgM antibodies with cytokine/chemokine levels during acute Chikungunya virus infection
Autor(es): Reddy Vijayalakshmi, Mani Reeta Subramaniam, Desai Anita, Ravi Vasanthapuram
Resumo: Chikungunya (CHIKV) is an emerging arboviral infection of public health concern in India contributing to widespread morbidity. The precise molecular events occurring early in the infection have not been well understood. Cytokines/chemokines are suspected to play a key role in its pathogenesis. Very few studies have correlated the plasma levels of cytokines/chemokines with diagnostic markers such as viral loads - presence of CHIKV IgM antibodies. Underst-ing these dynamics in the early phase of CHIKV infection is likely to provide an insight into the evolution of the immune response, identify biomarkers for assessing severity, - for development of newer therapeutic strategies. This study was therefore undertaken to estimate the levels of various cytokines/chemokines in plasma samples of patients infected with CHIKV - correlate to viral load - CHIKV IgM antibodies. Cytokine/chemokine levels - viral loads in plasma were measured using cytometric bead array - TaqMan real time PCR assay, respectively. The findings revealed that acute phase of CHIKV infection is characterized by predominant inflammatory responses mediated by IL-6, IL-8, IP-10, MCP-1, - MIG (P < 0.003). Plasma levels of IL-6 (r = 0.53, P < 0.05) - MCP-1 (r = 0.83, P < 0.05) emerged as reliable biomarkers of high viral loads in Chikungunya patients. Further, presence of elevated levels of MCP-1 - MIG during the chronic phase of the disease suggests that these chemokines may contribute to perpetuation of symptoms. Hence, these chemokines might serve as targets for the development of treatment to ameliorate the symptoms during the acute phase - prevent the development of chronic manifestations.
Palavras-Chave: Biomarkers of Chikungunya severity; Cytokines/chemokines in Chikungunya
Imprenta: Journal of Medical Virology, v. 86, n. 8, p. 1393-1401, 2014
Identificador do objeto digital: 10.1002/jmv.23875
Descritores: Chikungunya virus - Immune response ; Chikungunya virus - Pathogenesis ; Chikungunya virus - Proteins ; Chikungunya virus - Antibodies ; Chikungunya virus - Cytokines ; Chikungunya virus - Immune response ; Chikungunya virus - Viral infections ; Chikungunya virus - Real Time PCR ; Chikungunya Virus - Virus ; Chikungunya virus - Chikungunya fever ; Chikungunya virus - Immunology ; Chikungunya virus - Public health
Data de publicação: 2014
