Autor(es): Wikan Nitwara, Khongwichit Sarawut, Phuklia Weerawat, Ubol Sukathida, Thonsakulprasert Tipparat, Thannagith Montri, Tanramluk Duangrudee, Paemanee Atchara, Kittisenachai Suthathip, Roytrakul Sittiruk, Smith Duncan R

Resumo: Chikungunya fever (CHIKF) is a recently re-emerged mosquito transmitted viral disease caused by the chikungunya virus (CHIKV), an Alphavirus belonging to the family Togaviridae. Infection of humans with CHIKV can result in CHIKF of variable severity, although the factors mediating disease severity remain poorly defined. White blood cells were isolated from blood samples collected during the 2009-2010 CHIKF outbreak in Thail-. Clinical presentation - viral load data were used to classify samples into three groups, namely non chikungunya fever (non-CHIKF), mild CHIKF, - severe CHIKF. Five samples from each group were analyzed for protein expression by GeLC-MS/MS. CHIKV proteins (structural - non-structural) were found only in CHIKF samples. A total of 3505 human proteins were identified, with 68 proteins only present in non-CHIKF samples. A total of 240 proteins were found only in CHIKF samples, of which 65 - 46 were found only in mild - severe CHIKF samples respectively. Proteins with altered expression mapped predominantly to cellular signaling pathways (including toll-like receptor - PI3K-Akt signaling) although many other processes showed altered expression as a result of CHIKV infection. Expression of proteins consistent with the activation of the inflammasome was detected, - quantitation of (pro)-caspase 1 at the protein - RNA levels showed an association with disease severity. This study confirms the infection of at least a component of white blood cells by CHIKV, - shows that CHIKV infection results in activation of the inflammasome in a manner that is associated with disease severity.

Palavras-Chave: Chikungunya; Proteome; Inflammasome; Caspase 1

Imprenta: Journal of Translational Medicine, v. 12, p. 96, 2014

Identificador do objeto digital: 10.1186/1479-5876-12-96

Descritores: Chikungunya virus - Biochemistry ; Chikungunya virus - Biosynthesis ; Chikungunya virus - Cell ; Chikungunya virus - DNA ; Chikungunya virus - Molecular structure ; Chikungunya virus - Proteins ; Chikungunya virus - RNA ; Chikungunya virus - Viral infections ; Chikungunya virus - Molecular methods ; Chikungunya virus - Real Time PCR ; Chikungunya Virus - Virus ; Chikungunya virus - Chikungunya fever

Data de publicação: 2014

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