Clinical features and molecular diagnosis of Chikungunya fever from South India
Autor(es): Lakshmi Vemu, Neeraja Mamidi, Subbalaxmi M V S, Parida M M, Dash P K, Santhosh S R, Rao P V L
Resumo: An epidemic of Chikungunya fever of unprecedented magnitude occurred in many parts of India in early 2006 after an interval of 33 years, - there has been a resurgence in some parts of South India since June 2007. The article highlights clinical manifestations of infection - various molecular tests that were used for diagnoses of Chikungunya virus infection. Of particular interest is the real-time loop-mediated isothermal amplification (RT LAMP) assay, which is rapid - cost-effective - can be adopted at ill-equipped laboratories. Clinical symptoms were characterized by a triad of fever, rash, - severe rheumatic manifestations. RT LAMP identified 20 additional Chikungunya virus-positive cases, compared with reverse-transcriptase polymerase chain reaction. Chikungunya virus was isolated from 20 r-omly selected samples. Genotyping of the virus isolates revealed that the East Central South African genotype of Chikungunya virus was the etiologic agent of this epidemic. Molecular diagnosis is an important tool to identify such new vectorborne viral illnesses.
Imprenta: Clinical Infectious Diseases, v. 46, n. 9, p. 1436-1442, 2008
Identificador do objeto digital: 10.1086/529444
Descritores: Chikungunya virus - Cytopathology ; Chikungunya virus - Pathogenesis ; Chikungunya virus - Viral infections ; Chikungunya virus - Molecular methods ; Chikungunya virus - Real Time PCR ; Chikungunya virus - RT-PCR ; Chikungunya Virus - Virus ; Chikungunya virus - Molecular screening ; Chikungunya virus - Chikungunya fever ; Chikungunya virus - Epidemic ; Chikungunya virus - Public health
Data de publicação: 2008
