Autor(es): Pyndiah M N, Pursem V, Meetoo G, Daby S, Ramuth V, Bhinkah P, Chuttoo R, Paratian U

Resumo: During the chikungunya outbreak of 2005 - 2006, the only laboratory facilities available in Mauritius were virus isolation in cell culture tubes - serology. The laboratory was submerged with large numbers of blood samples. Comparative isolation was made in human embryonic lung (HEL) - VERO cells grown in 96-well plate. Culture on HEL cells was found to be more sensitive - presence of cytopathic effect (CPE) was observed earlier than in VERO cells. Out of the 18 300 blood samples inoculated on HEL, 11 165 were positive. This virus isolation method was of great help for the surveillance - control of the vectors. In cases of an outbreak a cheap, rapid - simple method of isolating chikungunya virus is described.

Imprenta: Me?decine Tropicale, v. 72, Spec No, p. 63-65, 2012

Descritores: Chikungunya virus - Cell ; Chikungunya virus - Pathogenesis ; Chikungunya virus - Serology ; Chikungunya virus - Viral infections ; Chikungunya virus - Serology ; Chikungunya Virus - Virus ; Chikungunya virus - Chikungunya fever ; Chikungunya virus - Epidemiology

Data de publicação: 2012

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