Autor(es): Roy Chad J, Adams A Paige, Wang Eryu, Plante Kenneth, Gorchakov Rodion, Seymour Robert L, Vinet-Oliphant Heather, Weaver Scott C

Resumo: Chikungunya virus (CHIKV) is a mosquito-borne alphavirus that causes major epidemics of rash, fever, - debilitating arthritis. Currently, there are no vaccines or antivirals available for prevention or treatment. We therefore generated 2 live-attenuated vaccine c-idates based on the insertion of a picornavirus internal ribosome entry site (IRES) sequence into the genome of CHIKV. Vaccination of cynomolgus macaques with a single dose of either vaccine produced no signs of disease but was highly immunogenic. After challenge with a subcutaneous inoculation of wild-type CHIKV, both vaccine c-idates prevented the development of detectable viremia. Protected animals also exhibited no significant changes in core body temperature or cardiovascular rhythm, whereas sham-vaccinated animals showed hyperthermia, followed by sustained hypothermia, as well as significant changes in heart rate. These CHIKV/IRES vaccine c-idates appear to be safe - efficacious, supporting their strong potential as human vaccines to protect against CHIKV infection - reduce transmission - further spread.

Palavras-Chave: Chikungunya virus; Nonhuman primates; Viral vaccine

Imprenta: The Journal of Infectious Diseases, v. 209, n. 12, p. 1891-1899, 2014

Identificador do objeto digital: 10.1093/infdis/jiu014

Descritores: Chikungunya virus - Cytopathology ; Chikungunya virus - Genome ; Chikungunya virus - Pathogenesis ; Chikungunya virus - Proteins ; Chikungunya virus - Antibodies ; Chikungunya virus - Viral infections ; Chikungunya Virus - Virus ; Chikungunya virus - Transmission ; Chikungunya virus - Vaccine ; Chikungunya virus - Chikungunya fever ; Chikungunya virus - Epidemic ; Chikungunya virus - Immunology ; Chikungunya virus - Public health

Data de publicação: 2014

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