Autor(es): Bourjot Mélanie, Leyssen Pieter, Eydoux Cécilia, Guillemot Jean-Claude, Canard Bruno, Rasoanaivo Philippe, Guéritte Françoise, Litaudon Marc

Resumo: In an effort to identify novel inhibitors of Chikungunya (CHIKV) - Dengue (DENV) virus replication, a systematic study with 820 ethyl acetate extracts of Madagascan plants was performed in a virus-cell-based assay for CHIKV - a DENV NS5 RNA-dependant RNA polymerase (RdRp) assay. The extract obtained from the leaves of Anacolosa pervilleana was selected for its significant activity in both assays. One new (E)-tridec-2-en-4-ynedioic acid named anacolosine (1), together with three known acetylenic acids, the octadeca-9,11,13-triynoic acid (2), (13E)-octadec-13-en-9,11-diynoic acid (3), (13E)-octadec-13-en-11-ynoic acid (4), two terpenoids, lupenone (5) - ?-amyrone (6), - one cyanogenic glycoside, (S)-sambunigrin (7) were isolated. Their structures were elucidated by comprehensive analyses of NMR spectroscopy - mass spectrometry data. The inhibitory potency of these compounds was evaluated on CHIKV, DENV RdRp - West-Nile polymerase virus (WNV RdRp). Both terpenoids showed a moderate activity against CHIKV (EC(50) 77 - 86 ?M, respectively) - the acetylenic acids produced IC(50) values around 3 ?M in the DENV RdRp assay.

Palavras-Chave: Anacolosa pervilleana; Acetylenic acid; Sambunigrin; Antiviral; Chikungunya virus; Dengue virus

Imprenta: Fitoterapia, v. 83, n. 6, p. 1076-1080, 2012

Identificador do objeto digital: 10.1016/j.fitote.2012.05.004

Descritores: Chikungunya virus - Biosynthesis ; Chikungunya virus - Cell ; Chikungunya virus - DNA ; Chikungunya virus - Flaviviridae ; Chikungunya virus - Molecular structure ; Chikungunya virus - RNA ; Chikungunya virus - Viral infections ; Chikungunya Virus - Virus ; Chikungunya virus - Dengue

Data de publicação: 2012

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