Autor(es): Chua Chong Long, Chan Yoke Fun, Sam I-Ching

Resumo: Chikungunya virus (CHIKV) is a mosquito-borne arbovirus which has recently re-emerged globally - poses a major threat to public health. Infection leads to severe arthralgia, - disease management remains supportive in the absence of vaccines - anti-viral interventions. The high specificities of monoclonal antibodies (mAbs) have been exploited in immunodiagnostics - immunotherapy in recent decades. In this study, eight different clones of mAbs were generated - characterised. These mAbs targeted the linear epitopes on the CHIKV E2 envelope glycoprotein, which is the major target antigen during infection. All the mAbs showed binding activity against the purified CHIKV virion or recombinant E2 when analysed by immunofluorescence, ELISA - Western blot. The epitopes of each mAb were mapped by overlapping synthetic peptide-based ELISA. The epitopes are distributed at different functional domains of E2 glycoprotein, namely at domain A, junctions of ?-ribbons with domains A - B, - domain C. Alignment of mAb epitope sequences revealed that some are well-conserved within different genotypes of CHIKV, while some are identical to - likely to cross-react with the closely-related alphavirus O'nyong-nyong virus. These mAbs with their mapped epitopes are useful for the development of diagnostic or research tools, including immunofluorescence, ELISA - Western blot.

Palavras-Chave: Alphaviruses; Chikungunya virus; E2 glycoprotein; Linear epitopes; Monoclonal antibody; Recombinant proteins

Imprenta: Journal of Virological Methods, v. 195, p. 126-133, 2014

Identificador do objeto digital: 10.1016/j.jviromet.2013.10.015

Descritores: Chikungunya virus - Arbovirus ; Chikungunya virus - Pathogenesis ; Chikungunya virus - Proteins ; Chikungunya virus - Antibodies ; Chikungunya Virus - Virus ; Chikungunya virus - Vaccine ; Chikungunya virus - Immunology ; Chikungunya virus - Public health

Data de publicação: 2014

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