Association of Oligoadenylate Synthetase Gene Cluster and DC-SIGN (CD209) Gene Polymorphisms with Clinical Symptoms in Chikungunya Virus Infection
Autor(es): Chaaithanya Itta Krishna, Muruganandam Nagarajan, Surya Palani, Anwesh Maile, Alagarasu Kalichamy, Vijayachari Paluru
Resumo: Biology and pathogenesis of chikungunya virus (CHIKV) are not clearly established. Host factors play an important role in determining the progression - severity of the disease. Polymorphisms in the promoter region of CD209 gene (rs735239, rs4804803, rs2287886) - OAS1 (rs1131454 - rs10774671), OAS2 (rs15895 - rs1732778), - OAS3 (rs2285932 - rs2072136) genes were investigated in 100 patients with CHIKV infection - 101 healthy controls to find out the association of these polymorphisms with CHIKV infection. To evaluate the association of OAS - CD209 gene polymorphisms with the presence or absence of disease symptoms in CHIKV-infected patients. DNA was extracted - typed using polymerase chain reaction followed by restriction fragment length polymorphism methods. Results revealed that the allele - genotype frequencies of OAS1, OAS3, - OAS2 gene polymorphisms were not different between healthy controls - CHIKV patients. The frequency of CD209 gene G/G genotype of rs4804803 was significantly higher in CHIKV patients compared to healthy controls (p = 0.046). The present study suggests that rs4804803 GG genotype of CD209 gene is associated with susceptibility to CHIKV infection. To conclude, the present preliminary study suggests that OAS gene cluster - CD209 gene polymorphisms influence the risk of developing clinical symptoms in CHIKV-infected patients. Further follow-up studies with a large number of samples are needed to assess the role of these genes in association with post-sequela symptoms observed in CHIKV patients. A detailed research is required in these directions to underst- the biology behind CHIKV infection - disease severity.
Imprenta: DNA and Cell Biology, v. 35, n. 1, p. 44-50, 2016
Identificador do objeto digital: 10.1089/dna.2015.2819
Descritores: Chikungunya virus - Cell ; Chikungunya virus - DNA ; Chikungunya virus - Pathogenesis ; Chikungunya Virus - Virus ; Chikungunya virus - Dengue
Data de publicação: 2016
