Assessment of packed bed bioreactor systems in the production of viral vaccines
Autor(es): Rajendran Ramya, Lingala Rajendra, Vuppu Siva Kumar, Bandi Bala Obulapathi, Manickam Elaiyaraja, Macherla Sankar Rao, Dubois Stéphanie, Havelange Nicolas, Maithal Kapil
Resumo: Vaccination is believed to be the most effective method for the prevention of infectious diseases. Thus it is imperative to develop cost effective - scalable process for the production of vaccines so as to make them affordable for mass use. In this study, performance of a novel disposable iCELLis fixed bed bioreactor system was investigated for the production of some viral vaccines like Rabies, Hepatitis-A - Chikungunya vaccines in comparison to conventional systems like the commercially available packed bed system - roller bottle system. Vero - MRC-5 cell substrates were evaluated for growth parameters in all the three systems maintaining similar seeding density, multiplicity of infection (MOI) - media components. It was observed that Vero cells showed similar growth in all the three bioreactors whereas MRC-5 cells showed better growth in iCELLis Nano system - roller bottle system. Subsequently, the virus infection - antigen production studies also revealed that for Hepatitis-A - Chikungunya iCELLis Nano bioreactor system was better to the commercial packed bed bioreactor - roller bottle systems. Although for rabies antigen production commercially available packed bed bioreactor system was found to be better. This study shows that different bioreactor platforms may be employed for viral vaccine production - iCELLis Nano is one of such new convenient - a stable platform for production of human viral vaccines.
Palavras-Chave: Chikungunya virus; Hepatitis-A virus; Packed bed bioreactor; Rabies virus; Vaccine production; iCELLis Nano bioreactor
Imprenta: AMB Express, v. 4, p. 25, 2014
Identificador do objeto digital: 10.1186/s13568-014-0025-z
Descritores: Chikungunya virus - Cell ; Chikungunya virus - Infectious diseases ; Chikungunya virus - Viral infections ; Chikungunya Virus - Virus ; Chikungunya virus - Vaccine
Data de publicação: 2014
