Autor(es): Wang Ruoxing, Wang Jundi, Acharya Dhiraj, Paul Amber M, Bai Fengwei, Huang Faqing, Guo Yan-Lin

Resumo: We have recently reported that mouse embryonic stem cells (mESCs) are deficient in expressing type I interferons (IFNs) in response to viral infection - synthetic viral RNA analogs (Wang, R., Wang, J., Paul, A. M., Acharya, D., Bai, F., Huang, F., - Guo, Y. L. (2013) J. Biol. Chem. 288, 15926-15936). Here, we report that mESCs are able to respond to type I IFNs, express IFN-stimulated genes, - mediate the antiviral effect of type I IFNs against La Crosse virus - chikungunya virus. The major signaling components in the IFN pathway are expressed in mESCs. Therefore, the basic molecular mechanisms that mediate the effects of type I IFNs are functional in mESCs; however, these mechanisms may not yet be fully developed as mESCs express lower levels of IFN-stimulated genes - display weaker antiviral activity in response to type I IFNs when compared with fibroblasts. Further analysis demonstrated that type I IFNs do not affect the stem cell state of mESCs. We conclude that mESCs are deficient in type I IFN expression, but they can respond to - mediate the cellular effects of type I IFNs. These findings represent unique - uncharacterized properties of mESCs - are important for underst-ing innate immunity development - ESC physiology.

Palavras-Chave: Antiviral Response; Double-stranded RNA (dsRNA); Embryonic Stem Cell; Innate Immunity; Interferon; Interferon-stimulated Genes; Viral Replication

Imprenta: The Journal of Biological Chemistry, v. 289, n. 36, p. 25186-25198, 2014

Identificador do Objeto Digital: 10.1074/jbc.M113.537746

Descritores: Chikungunya virus - Biochemistry ; Chikungunya virus - Biosynthesis ; Chikungunya virus - Cell ; Chikungunya virus - Pathogenesis ; Chikungunya virus - Proteins ; Chikungunya virus - RNA ; Chikungunya virus - Cytokines ; Chikungunya virus - RT-PCR ; Chikungunya Virus - Virus ; Chikungunya virus - Immunology

Data de Publicação: 2014