Autor(es): Murali Krishnan Saravana, Sivasubramanian Srinivasan, Vincent Savariar, Murugan Shanmugaraj Bala, Giridaran Bupesh, Dinesh Sundaram, Gunasekaran Palani, Krishnasamy Kaveri, Sathishkumar Ramalingam

Resumo: To obtain luteolin - apigenin rich fraction from the ethanolic extract of Cynodon dactylon (L.) (C. dactylon) Pers - evaluate the fraction's cytotoxicity - anti-Chikungunya potential using Vero cells. The ethanolic extract of C. dactylon was subjected to silica gel column chromatography to obtain anti-chikungunya virus (CHIKV) fraction. Reverse phase-HPLC - GC-MS studies were carried out to identify the major phytochemicals in the fraction using phytochemical st-ards. Cytotoxicity - the potential of the fraction against CHIKV were evaluated in vitro using Vero cells. Reduction in viral replication was assessed by reverse transcriptase-polymerase chain reaction (RT-PCR) after treating the viral infected Vero cells with the fraction. Reverse Phase-HPLC - GC-MS studies confirmed the presence of flavonoids, luteolin - apigenin as major phytochemicals in the anti-CHIKV ethanolic fraction of C. dactylon. The fraction was found to exhibit potent viral inhibitory activity (about 98%) at the concentration of 50 µg/mL as observed by reduction in cytopathic effect, - the cytotoxic concentration of the fraction was found to be 250 µg/mL. RT-PCR analyses indicated that the reduction in viral mRNA synthesis in fraction treated infected cells was much higher than the viral infected control cells. Luteolin - apigenin rich ethanolic fraction from C. dactylon can be utilized as a potential therapeutic agent against CHIKV infection as the fraction does not show cytotoxicity while inhibiting the virus.

Palavras-Chave: Antiviral activity; Apigenin; Chikungunya virus; Cynodon dactylon; Flavonoids; Luteolin

Imprenta: Asian Pacific Journal of Tropical Medicine, v. 8, n. 5, p. 352-358, 2015

Identificador do objeto digital: 10.1016/S1995-7645(14)60343-6

Descritores: Chikungunya virus - Cell ; Chikungunya virus - RT-PCR ; Chikungunya Virus - Virus

Data de publicação: 2015

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