An animal model for studying the pathogenesis of Chikungunya virus infection
Autor(es): Ziegler Sarah A, Lu Liang, da Rosa Amelia P A Travassos, Xiao Shu-Yuan, Tesh Robert B
Resumo: Newborn - 14-day-old mice inoculated subcutaneously with chikungunya virus (CHIKV) developed lethargy, difficulty walking, dragging of hind limbs, - reduced weight gain within 7-10 days after infection (PI). During the initial 6-7 days PI, the animals had viremia; high levels (10(6)-10(8) PFU) of CHIKV were also present in leg muscle. The virus persisted in muscle for several days after viremia disappeared. The major histopathologic changes were in skeletal muscle, which were focal necrosis - inflammation, followed by fibrosis - dystrophic calcification. Some mice also showed dystrophic calcification in the joint cartilage, but there were few deaths, - most of the animals eventually recovered. CHIKV antigen was shown by immunohistochemistry in the muscle for several weeks after infection. Based on the clinical - pathologic similarities with CHIKV infection in humans, young ICR - CD-1 mice offer a useful - realistic model for further study of the pathogenesis - treatment of CHIKV infection.
Imprenta: The American Society of Tropical Medicine and Hygiene, v. 79, n. 1, p. 133-139, 2008
Descritores: Chikungunya virus - Cytopathology ; Chikungunya virus - Pathogenesis ; Chikungunya virus - Infectious diseases ; Chikungunya virus - Inflammation ; Chikungunya virus - Viral infections ; Chikungunya Virus - Virus
Data de publicação: 2008
