A novel 2006 Indian outbreak strain of chikungunya virus exhibits different pattern of infection as compared to prototype strain
Autor(es): Kumar Abhishek, Mamidi Prabhudutta, Das Indrani, Nayak Tapas K, Kumar Sameer, Chhatai Jagamohan, Chattopadhyay Subhasis, Suryawanshi Amol R, Chattopadhyay Soma
Resumo: With homologous pairs the adsorbed layer was 16.8 to 69. 8 mµ thick, usually 2, often 3 times as thick as that with heterologous pairs. The reactions with all heterologous sera were of the same order of magnitude, no intra-group cross-reactions were detected, unlike the results obtained with haemagglutinationinhibition and complement-fixation tests. From experiments reversing polarities, it was concluded that' antigen particles were positively charged at pH 7.0 and that antibody particles were negatively charged at pH 7.6. The method permitted the observation of specific adsorption of antibodies at extreme serum dilutions; layers 8.8 mµ and 4.0 mµ with homologous and heterologous serum pairs respectively were obtained at 10-5 dilution. By repetition of the procedure a dilute serum sample could be deprived of antibody and the method has possibilities for the quantitation of antibody content of sera. The results of the method were best at 20°C, rather than at 5°C or 35°C. Antigen-coated slides retained activity for 1 month at room temperature. The test requires only a few minutes to perform. The extension of the technique to other systems for general assessment is advised. W. H. R. Lumsden.
Imprenta: PloS One, v. 9, n. 1, p. e85714, 2014
Identificador do objeto digital: 10.1371/journal.pone.0085714
Descritores: Chikungunya virus - Cell ; Chikungunya virus - Genome ; Chikungunya virus - Pathogenesis ; Chikungunya virus - Proteins ; Chikungunya virus - RNA ; Chikungunya virus - Viral infections ; Chikungunya Virus - Virus ; Chikungunya virus - Chikungunya fever ; Chikungunya virus - Epidemic ; Chikungunya virus - Epidemiology ; Chikungunya virus - Public health
Data de publicação: 2014
