Proteome of Aedes aegypti in response to infection and coinfection with microsporidian parasites
Autor(es): Duncan, Alison B.; Agnew, Philip; Noel, Valerie; Demettre, Edith; Seveno, Martial; Brizard, Jean-Paul; Michalakis, Yannis
Resumo: Hosts are frequently infected with more than one parasite or pathogen at any one time, but little is known as to how they respond to multiple immune challenges compared to those involving single infections. We investigated the proteome of Aedes aegypti larvae following infection with either Edhazardia aedis or Vavraia culicis, and coinfections involving both. They are both obligate intracellular parasites belonging to the phylum microsporidia and infect natural populations of Ae. aegypti. The results found some proteins only showing modified abundance in response to infections involving E. aedis, while others were only differentially abundant when infections involved V. culicis. Some proteins only responded with modified abundance to the coinfection condition, while others were differentially abundant in response to all three types of infection. As time since infection increased, the response to each of the single parasite infections diverged, while the response to the E. aedis and coinfection treatments converged. Some of the proteins differentially abundant in response to infection were identified. They included two vacuolar ATPases, proteins known to have a role in determining the infection success of intracellular parasites. This result suggests microsporidia could influence the infection success of other intracellular pathogens infecting vector species of mosquito, including viruses, Plasmodium and Wolbachia. This study explores how simultaneous infection with two different microsporidian parasite species influences the proteome of the mosquito Aedes aegyptii, compared to single infections of each parasite. We show that the mosquito response is mostly specific to each type of infection with few proteins with unanimously modified abundance across all infection types. We identify two vacuolar ATPases, proteins known to have a role in determining the infection success of intracellular parasites. This result suggests Microsporidia could influence the infection success of other intracellular pathogens, such as Plasmodium or dengue, that are vectored by mosquitoes.
Palavras-Chave: Parasites; Viruses; Disease control; Natural populations; Pathogens; Public health; Disease transmission; Microsporidia; Dengue; Larvae; Vectors; Infection; Proteins; Plasmodium; Aedes aegypti; Microsporidia; Wolbachia; Edhazardia aedis
Imprenta: Ecology and Evolution, v. 2, n. 4, p. 681-694, 2012.
Descritores: Aedes aegypti - Immune response ; Aedes aegypti - Virus ; Aedes aegypti - Dengue
Data de publicação: 2012
