Autor(es)Khasdan, V.; Ben-Dov, E.; Manasherob, R.; Boussiba, S.; Zaritsky, A.

ResumoGenes encoding the mosquito larvicidal toxins Cry4Aa, Cry11Aa, Cyt1Aa and the regulatory P20 from Bacillus thuringiensis subsp. israelensis were introduced into the nitrogen-fixing, filamentous cyanobacterium Anabaena PCC 7120 for expression under control of two strong promoters PpsbA and PA1. The clone pRVE4-ADRC displayed toxicity against fourth-instar larvae of Aedes aegypti, the highest ever achieved in cyanobacteria. It was about 2.5-fold more toxic than the respective clone without cyt1Aa [Wu et al., Appl. Environ. Microbiol. 63 (1997) 4971-4975]. Cyt1Aa synergized the combination of Crys by about five-fold. Consistently, the lethal times exerted by pRVE4-ADRC were also reduced (it killed exposed larvae more quickly). This clone may become a useful biological control agent which reduces the probability of resistance development in the target organisms [Wirth et al., Proc. Natl. Acad. Sci. USA 94 (1997) 10536-10540].

Palavras-ChaveClones; Biological control; Population genetics; Toxicants; Pesticides; Phytoplankton; Pest control; Toxicity; Larval development; Synergism; Nitrogen fixation; Toxins; Larvicides

ImprentaFEMS Microbiology Letters, v. 227, n. 2, p. 189-195, 2003.

DescritoresAedes aegypti - Proteins ; Aedes aegypti - Larvicide

Data de Publicação:2003