Autor(es)Lee, D. H.; Cha, I. H.; Woo, D. S.; Ohba, M.

ResumoA total of 34 fecal samples, collected from 14 species of wild mammals in Korea, were examined for the occurrence of Bacillus thuringiensis. The organism was detected in 18 (53%) samples. Among the three food-habit groups, herbivorous animals yielded the highest frequency (69%) of samples positive for B. thuringiensis, followed by omnivorous animals (50%). Of the six fecal samples from carnivorous animals, only one sample contained B. thurin giensis. Among 527 isolates belonging to the Bacillus cereus-B. thuringiensis group, 43 (8%) were assigned to B. thurin giensis on the basis of the formation of parasporal inclusions. Of the 43 isolates, 13 were serologically allocated to the nine H-antigenic serotypes: H3ad (serovar sumiyoshiensis), H15 (dakota), H17/27 (tohokuensis/ mexicanensis), H19 (tochigiensis), H21 (colmeri), H29 (amagiensis), H31/49 (toguchini/muju), H42 (jinghongiensis), and H44 (higo). Other isolates were untestable or untypable by the 55 reference H antisera available. Insecticidal activity was associated with 23% of the fecal populations: three isolates killed larvae of the silkworm, Bombyx mori (Lepidoptera), and seven exhibited larvicidal activity against the mosquito, Aedes aegypti (Diptera). There was no larvicidal activity against the three lepidopterous insects: Plutella xylostella, Spodoptera exigua, and Spodoptera litura. The overall results suggest that wild animals in Korea are in contact with naturally occurring B. thuringiensis at high frequencies through the daily food intake of plants.Original Abstract: Un total de la 34 echantillons fecaux, recueillis a partir de la 14 especes de la mammiferes sauvages de la Coree, ont ete analyse afin de la detecter la presence de la Bacillus thuringiensis. L'organisme a ete detecte dans 18 (53 %) des echantillons. Parmi les trois groupes d'habitudes alimentaires, les herbivores ont donne la plus haute frequence d'echantillons positifs pour B. thuringiensis avec 69 %, suivis par les omnivores avec 50 %. Des dix echantillons fecaux de la carnivores, seulement un contenait du B. thuringiensis. Parmi les 527 isolats appartenant aux groupe Bacillus cereus-B. thuringiensis, 43 (8 %) ont ete classifies en tant que B. thuringiensis selon la formation d'inclusions parasporales. Des 43 isolats, 13 ont ete serotypes et classes dans les neuf serotypes H-antigeniques: H3ad (serovar sumiyoshiensis), H15 (dakota), H17/27 (tohokuensis/mexicanensis), H19 (tochigiensis), H21 (colmeri), H29 (amagiensis), H31/49 (toguchini/ muju), H42 (jinghongiensis), et H44 (higo). Les autres n'ont pu etre testes ou types par les 55 antiserums de la reference disponibles. Une activite insecticide a ete associee a 23 % des populations fecales : trois isolats ont tue des larves du ver a soie, Bombyx mori (Lepidoptera), et sept ont demontre un activite larvicide contre te moustique, Aedes aegypti (Diptera). Aucune activite larvicide n'a ete note contre les trois insectes lepidopteres: Plutella xylostella, Spodoptera exigua et Spodoptera litura. Pris dans leur ensemble, les resultats indiquent que les animaux sauvages de la Coree sont en contact frequent avec du B. thuringiensis d'origine naturelle a travers un regime quotidien de la plantes.

Palavras-ChaveBiological vectors; Biological control; Bacteria; Human diseases; Excretory products; Wildlife; Plants; Aedes aegypti; Bacillus thuringiensis;

ImprentaCanadian Journal of Microbiology, v. 49, n. 7, p. 465-471, 2003.

DescritoresAedes aegypti - Larvicide

Data de Publicação:2003