Autor(es): Khasdan, V; Ben-Dov, E; Manasherob, R; Boussiba, S; Zaritsky, A

Resumo: Genes encoding the mosquito larvicidal toxins Cry4Aa, Cry11Aa, Cyt1Aa and the regulatory P20 from Bacillus thuringiensis subsp. israelensis were introduced into the nitrogen-fixing, filamentous cyanobacterium Anabaena PCC 7120 for expression under control of two strong promoters P(psbA) and P(A1). The clone pRVE4-ADRC displayed toxicity against fourth-instar larvae of Aedes aegypti, the highest ever achieved in cyanobacteria. It was about 2.5-fold more toxic than the respective clone without cyt1Aa [Wu et al., Appl. Environ. Microbiol. 63 (1997) 4971-4975]. Cyt1Aa synergized the combination of Crys by about five-fold. Consistently, the lethal times exerted by pRVE4-ADRC were also reduced (it killed exposed larvae more quickly). This clone may become a useful Biological Control agent which reduces the probability of resistance development in the target organisms. (C) 2003 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.

Palavras-Chave: Mosquitocidal Anabaena; Delta-Endotoxin; Bacillus Thuringiensis Subsp Israelensis

Imprenta: Fems Microbiology Letters, v. 227, n. 2, p. 189-195, 2003

Identificador do objeto digital: 10.1016/S0378-1097(03)00679-7

Descritores: Aedes aegypti - RNA

Data de publicação: 2003