Mosquito densonucleosis virus non-structural protein NS2 is necessary for a productive infection
Autor(es): Azarkh, Eugene; Robinson, Erin; Hirunkanokpun, Supanee; Afanasiev, Boris; Kittayapong, Pattamaporn; Carlson, Jonathan; Corsini, Joe
Resumo: Mosquito densonucleosis viruses synthesize two non-structural proteins, NS1 and NS2. While NS1 has been studied relatively well, little is known about NS2. Antiserum was raised against a peptide near the N-terminus of NS2, and used to conduct Western blot analysis and immunofluorescence assays. Western blots revealed a prominent band near the expected size (41 kDa). Immuno-fluorescence studies of mosquito cells transfected with AeDNV indicate that NS2 has a wider distribution pattern than does NS 1, and the distribution pattern appears to be a function of time post-infection. Nuclear localization of NS2 requires intact C-terminus but does not require additional viral proteins. Mutations ranging from complete NS2 knock-out to a single missense amino acid substitution in NS2 can significantly reduce viral replication and production of viable progeny. (c) 2007 Elsevier Inc. All rights reserved.
Palavras-Chave: Mosquito Densonucleosis Viruses; Non-Structural Proteins; Antibodies; Western Analysis; Immuno-Fluorescent Assay
Imprenta: Virology, v. 374, n. 1, p. 128-137, 2008
Identificador do objeto digital: 10.1016/j.virol.2007.11.035
Descritores: Aedes aegypti - Cell ; Aedes aegypti - DNA ; Aedes aegypti - Viral infections ; Aedes aegypti - Virus
Data de publicação: 2008
