Autor(es)Sonoda, S; Maruyama, T; Izumi, Y; Yoshida, H; Tsumuki, H

ResumoChilo suppressalis transferrin cDNA was cloned by reverse transcription polymerase chain reaction (RT-PCR). The clone (2,248 bp) encodes a protein of 644 amino acids with a calculated molecular weight of 71,805 Da. The deduced amino acid Sequence showed significant homology with known insect transferrins from Bombyx mori (82%), Manduca sexta (77%), Blaberus discoidalis (43%), Riptortus clavatus (42%), Aedes aegypti (36%), Sarcophaga peregrina (32%), Drosophila melanogaster (33%) and D. silvestris (32%). Transferrin expression was examined by RNA gel blot analysis using diapausing and non-diapausing larvae of C. suppressalis. As a result, higher levels of expression were observed in diapausing larvae.

Palavras-ChaveLarval Diapause; The Rice Stem Borer; Transferrin

ImprentaApplied Entomology and Zoology, v. 39, n. 3, p. 463-468, 2004

DescritoresAedes aegypti - DNA ; Aedes aegypti - RNA ; Aedes aegypti - RT-PCR

Data de Publicação:2004