Molecular cloning, nucleotide Sequence and gene expression of a transferrin gene from the rice stem borer, Chilo suppressalis Walker (Lepidoptera : Crambidae)
Autor(es): Sonoda, S; Maruyama, T; Izumi, Y; Yoshida, H; Tsumuki, H
Resumo: Chilo suppressalis transferrin cDNA was cloned by reverse transcription polymerase chain reaction (RT-PCR). The clone (2,248 bp) encodes a protein of 644 amino acids with a calculated molecular weight of 71,805 Da. The deduced amino acid Sequence showed significant homology with known insect transferrins from Bombyx mori (82%), Manduca sexta (77%), Blaberus discoidalis (43%), Riptortus clavatus (42%), Aedes aegypti (36%), Sarcophaga peregrina (32%), Drosophila melanogaster (33%) and D. silvestris (32%). Transferrin expression was examined by RNA gel blot analysis using diapausing and non-diapausing larvae of C. suppressalis. As a result, higher levels of expression were observed in diapausing larvae.
Palavras-Chave: Larval Diapause; The Rice Stem Borer; Transferrin
Imprenta: Applied Entomology and Zoology, v. 39, n. 3, p. 463-468, 2004
Identificador do objeto digital: 10.1303/aez.2004.463
Descritores: Aedes aegypti - DNA ; Aedes aegypti - RNA ; Aedes aegypti - RT-PCR
Data de publicação: 2004
