Autor(es): Cheon, HM; Seo, SJ; Sun, JX; Sappington, TW; Raikhel, AS

Resumo: Lipophorin (Lp) functions as a yolk protein precursor in the mosquito Aedes aegypti and it is internalized via receptor-mediated endocytosis (Insect Biochem. Mel. Biol., 30 (2000) 1161). We cloned and molecularly characterized a putative mosquito ovarian lipophorin receptor (AaLpRov) cDNA. The cDNA has a length of 3468 bp coding for a 1156-residue protein with a predicted molecular mass of 128.9 kDa. The deduced amino acid Sequence of the cDNA revealed that it encodes a protein homolog of the LDL receptor superfamily, and that it harbors eight cysteine-rich ligand binding repeats at the N-terminus like vertebrate VLDL receptors. The deduced amino acid Sequence of this mosquito ovarian receptor is most similar to that of the locust lipophorin receptor (LmLpR) (64.3%), and is only distantly related to the mosquito vitellogenin receptor (VgR) (18.3%), another ovarian LDLR homolog with a different ligand. The AaLpRov cDNA was expressed in a TnT(R) Coupled Reticulocyte Lysate system, and coimmunoprecipitation experiments confirmed that the receptor protein specifically binds Lp. Developmental expression profiles clearly showed that AaLpRov transcripts are present in the vitellogenic ovary, with peak expression at 24-36 h post blood meal. In situ hybridization indicated that AaLpRov transcripts are present only in female germ line cells. Distance-based phylogenetic analyses suggest that the insect LpR and vertebrate LDL/VLDL receptor lineages separated after divergence from the insect VgR lineage. (C) 2001 Elsevier Science Ltd. All rights reserved.

Palavras-Chave: Mosquito; Lipophorin Receptor; Egg Development; Receptor-Mediated Endocytosis; Low Density Lipoprotein Receptor Family

Imprenta: Insect Biochemistry and Molecular Biology, v. 31, n. 8, p. 753-760, 2001

Identificador do objeto digital: 10.1016/S0965-1748(01)00068-6

Descritores: Aedes aegypti - Cell ; Aedes aegypti - DNA

Data de publicação: 2001

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