Molecular characterization of the cDNA encoding prophenoloxidase-2 (PPO2) and its expression in diamondback moth Plutella xylostella
Autor(es): Du, Lei; Li, Bin; Gao, Li; Xue, Chao-Bin; Lin, Jin; Luo, Wan-Chun
Resumo: Phenoloxidase (PO) is a key enzyme in the development process of insects. The cDNA of Plutella xylostella prophenoloxidase-2 (PxPPO2) was cloned by means of reverse transcription polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends (RACE). The full-length cDNA consisted of 2546 bp, containing an open reading frame (ORF) of 2091 bp which encoding 696 amino acids. BLASTp search and neighbor-joining analysis showed that the deduced amino acid Sequence had a high identity to the published Sequence of PPO2 from other lepidopterous insects, ranging from 72.2% to 78.3%. Semi-quantitative RT-PCR and real-time PCR indicated that the highest amount of PxPPO2 transcripts in eggs or the 4th instar larvae, followed by the 2nd, the 3rd instar larvae, prepupae, and pupa. Real-time PCR analysis showed that when the 3rd instar larvae treated with quercetin or kojic acid, the transcript levels of PPO2 became significantly decreased as compared with control ones. Crown Copyright (C) 2010 Published by Elsevier Inc. All rights reserved.
Palavras-Chave: Plutella xylostella; Prophenoloxidase; Melanization; Insect immunity; Real-time PCR
Imprenta: Pesticide Biochemistry and Physiology, v. 98, n. 2, p. 158-167, 2010
Identificador do objeto digital: 10.1016/j.pestbp.2010.04.011
Descritores: Aedes aegypti - RT-PCR ; Aedes aegypti - Real Time PCR
Data de publicação: 2010
