Autor(es): Steel, J. Jordan; Henderson, Brittney R.; Lama, Siddhi B. C.; Olson, Ken E.; Geiss, Brian J.

Resumo: We have developed a new method for producing infectious double subgenomic alphaviruses from plasmids transfected into mammalian cells. A double subgenomic Sindbis virus (TE3'2J) was transcribed from a cytomegalovirus PolII promoter, which results in the production of infectious virus. Transfection of as little as 125 ng of plasmid is able to produce 1 x 10(8) plaque forming units/ml (PFU/ml) of infectious virus 48 hours post-transfection. This system represents a more efficient method for producing recombinant Sindbis viruses. promoter,

Palavras-Chave: Sindbis; Alphavirus; Virus production; Gateway

Imprenta: Virology Journal, v. 8, 2011

Identificador do objeto digital: 10.1186/1743-422X-8-356

Descritores: Aedes aegypti - Cell ; Aedes aegypti - RNA ; Aedes aegypti - Virus

Data de publicação: 2011