Infectious alphavirus production from a simple plasmid transfection
Autor(es)Steel, J. Jordan; Henderson, Brittney R.; Lama, Siddhi B. C.; Olson, Ken E.; Geiss, Brian J.
ResumoWe have developed a new method for producing infectious double subgenomic alphaviruses from plasmids transfected into mammalian cells. A double subgenomic Sindbis virus (TE3'2J) was transcribed from a cytomegalovirus PolII promoter, which results in the production of infectious virus. Transfection of as little as 125 ng of plasmid is able to produce 1 x 10(8) plaque forming units/ml (PFU/ml) of infectious virus 48 hours post-transfection. This system represents a more efficient method for producing recombinant Sindbis viruses. promoter,
Palavras-ChaveSindbis; Alphavirus; Virus production; Gateway
ImprentaVirology Journal, v. 8, 2011
Identificador do Objeto Digital10.1186/1743-422X-8-356
DescritoresAedes aegypti - Cell ; Aedes aegypti - RNA ; Aedes aegypti - Virus
Data de Publicação:2011
