Autor(es): Paiva, Marcelo H. S.; Lovin, Diane D.; Mori, Akio; Melo-Santos, Maria A. V.; Severson, David W.; Ayres, Constancia F. J.

Resumo: Organophosphate insecticides (OP) have extensively been used to control mosquitoes, such as the vector Aedes aegypti. Unfortunately, OP resistance has hampered control programs worldwide. We used Quantitative Trait Locus (QTL) mapping to evaluate temephos resistance in two F-1 intercross populations derived from crosses between a resistant Ae. aegypti strain (RecR) and two susceptible strains (MoyoD and Red). A single major effect QTL was identified on chromosome 2 of both segregating populations, named rtt1 (resistance to temephos 1). Bioinformatics analyses identified a cluster of carboxylesterase genes (CCE) within the rtt1 interval. qRT-PCR demonstrated that different CCEs were up-regulated in F-2 resistant individuals from both crosses. However, none exceeded the 2-fold expression. Primary mechanisms for temephos resistance may vary between Ae. aegypti populations, yet also appear to support previous findings suggesting that multiple linked esterase genes may contribute to temephos resistance in the RecR strain as well as other populations. (C) 2015 Elsevier Inc. All rights reserved.

Palavras-Chave: Insecticide Resistance; Organophosphate Resistance; QTL; Aedes Aegypti; Carboxylesterases

Imprenta: Genomics, v. 107, n. 1, p. 40-48, 2016

Identificador do objeto digital: 10.1016/j.ygeno.2015.11.004

Descritores: Aedes aegypti - Public health

Data de publicação: 2016

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