Autor(es): Coy, M. R.; Tu, Z.

Resumo: Tango is a transposon of the TO family and was originally discovered in the African malaria mosquito, Anopheles gambiae. Here we report a systematic analysis of the genome Sequence of the yellow fever mosquito, Aedes aegypti, which uncovered three distinct Tango transposons. We name the only An. gambiae Tango transposon AgTango1 and the three Ae. aegypti Tango elements AeTango1-3. Like AgTangol, AeTango1 and AeTango2elements both have members that retain characteristics of autonomous elements such as intact open reading frames and terminal inverted repeats (TIRs). AeTango3 is a degenerate transposon with no full-length members. All full-length Tango transposons contain subterminal direct repeats within their TIRs. AgTangol and AeTango1-3form a single clade among other TO transposons. Within this clade, AgTangol and AeTango1 are closely related and share approximately 80% identity at the amino acid level, which exceeds the level of similarity of the majority of host genes in the two species. A survey of Tango in other mosquito species was carried out using degenerate PCR. Tango was isolated and Sequenced in all members of the An. gambiae species complex, Aedes albopictus and Ochlerotatus atropalpus. Oc. atropalpus contains a rich diversity of Tango elements, while Tango elements in Ae. albopictus and the An. gambiae species complex all belong to Tango1. No Tango was detected in Culex pipiens quinquefasciatus, Anopheles stephensi, Anopheles dirus, Anopheles farauti or Anopheles albimanus using degenerate PCR. Bioinformatic searches of the Cx. p. quinquefasciatus (similar to 10 x coverage) and An. stephensi (0.33 x coverage) databases also failed to uncover any Tango elements. Although other Evolutionary scenarios cannot be ruled out, there are indications that Tango1 underwent horizontal transfer among divergent mosquito species.

Palavras-Chave: Evolution; Horizontal Transfer; Interspersed Repeat; Mosquito; Transposable Element

Imprenta: Insect Molecular Biology, v. 16, n. 4, p. 411-421, 2007

Identificador do objeto digital: 10.1111/j.1365-2583.2007.00735.x

Descritores: Aedes aegypti - Genome

Data de publicação: 2007

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