Genetic transformation of the codling moth, Cydia pomonella L., with piggyBac EGFP
Autor(es): Ferguson, Holly J.; Neven, Lisa G.; Thibault, Stephen T.; Mohammed, Ahmed; Fraser, Malcolm
Resumo: Genetic transformation of the codling moth, Cydia pomonella, was accomplished through embryo microinjection with a plasmid-based piggyBac vector containing the enhanced green fluorescent protein (EGFP) gene. Sequencing of the flanking regions around the inserted construct resulted in identification of insect genomic Sequences, not plasmid Sequences, thus providing evidence that the piggyBac EGFP cassette had integrated into the codling moth genome. EGFP-positive moths were confirmed in the 28th and earlier generations post injection through PCR and Southern blot analyses, indicating heritability of the transgene.
Palavras-Chave: Codling moth; Sterile insect technique; Autocidal Biological Control; Genetic sexing strain
Imprenta: Transgenic Research, v. 20, n. 1, p. 201-214, 2011
Identificador do objeto digital: 10.1007/s11248-010-9391-8
Descritores: Aedes aegypti - Genome ; Aedes aegypti - Proteins ; Aedes aegypti - PCR detection
Data de publicação: 2011
