Frequency of V1016I and F1534C mutations in the voltage-gated sodium channel gene in Aedes aegypti in Venezuela
Autor(es): Alvarez, Leslie C.; Ponce, Gustavo; Saavedra-Rodriguez, Karla; Lopez, Beatriz; Flores, Adriana E.
Resumo: The V1016I and F1534C mutations in the voltage-gated sodium channel gene have been associated with resistance to pyrethroids and DDT in Aedes aegypti mosquitoes. A study was carried out to determine the frequency of I1016 and C1534 by real-time PCR in five natural populations of Ae. aegypti in Venezuela during 2008, 2010 and 2012, as well as in a strain selected with 0.14 mu g of deltamethrin for 15 generations.RESULTSIn natural populations, frequencies of I1016 varied between 0.01 and 0.37, and frequencies of C1534 between 0.35 and 1.0. In the Pampanito strain, the frequency of I1016 increased from 0.02 in F-1 up to 0.5 in F-15 and from 0.35 up to fixation for C1534 after selection with deltamethrin.CONCLUSIONThe results showed that C1534 frequencies are higher than I1016 frequencies in natural populations of Ae. aegypti in Venezuela, and that deltamethrin selected the C1534 more rapidly than I1016. (c) 2014 Society of Chemical Industry
Palavras-Chave: Kdr; I1016; C1534; Deltamethrin
Imprenta: Pest Management Science, v. 71, n. 6, p. 863-869, 2015
Identificador do objeto digital: 10.1002/ps.3846
Descritores: Aedes aegypti - Real Time PCR
Data de publicação: 2015
