Transfer and expression of the cryIVB and cryIVD genes of bacillus-thuringiensis subsp israelensis in Bacillus-sphaericus 2297

Autor(es): Poncet, S.; Delecluse, A.; Anello, G.; Klier, A.; Rapoport, G.


Resumo: The genes encoding the CryIVB and CryIVD crystal polypeptides of B. thuringiensis subsp. israelensis were cloned independently on a stable shuttle vector, and transferred into B. sphaericus 2297. Recombinant cells expressed the B. thuringiensis toxins during sporulation and were shown to be toxic to Aedes aegypti fourth instar larvae, whereas the parental strain was not.


Palavras-Chave: Bacillus sphaericus; Bacillus thuringiensis subsp, israelensis toxins; Larvicidal activity; Gene transfer; Gene expression


Imprenta: Fems Microbiology Letters, v. 117, n. 1, p. 91-95, 1994


Descritores: Aedes aegypti - Cell


Data de publicação: 1994