Autor(es): Bogdanova, ES

Resumo: Electrophoretic mobility of histones from cell cultures of Drosophila melanogaster and of the mosquito Aedes aegypti was determined in polyacrylamide gels in the presence of different concentrations of urea. Great similarity in the electrophoretic behavior of H3, H2A, H2B and H4 histones from the two insect species was found. Histone H1 of Aedes under all conditions tested had a markedly higher electrophoretic mobility than H1 of Drosophila, but differed only slightly from H1 histones of mouse and of hamster. As can be deduced from the mobility of Aedes H1 in the presence of sodium dodecyl sulphate its molecular weight is smaller than that of Drosophila H1 and is very close to the molecular weight of the main component of mouse H1 histone. Heterogeneity of the H1 histone from Drosophila is demonstrated. This heterogeneity is due to phosphorylation of a part of H1 molecules, since it disappears after the treatment of H1 preparations by alkaline phosphatase. Phosphorylated components were not found in the H1 of Aedes. Thus two representatives of Diptera, Aedes and Drosophila possessing polytene chromosomes at the larval stage of development have H1 histones with markedly different primary structures. This pact demonstrates that the polytenization of chromosomes may occur in species with markedly different H1 histones.

Imprenta: Molecular Biology Reports, v. 3, n. 5, p. 361-369, 1977

Identificador do objeto digital: 10.1007/BF00420395

Descritores: Aedes aegypti - Cell

Data de publicação: 1977

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