Cloning and characterization of 60S ribosomal protein L22 (RPL22) from Culex pipiens pallens
Autor(es): He, Ji; Sun, Haibo; Zhang, Donghui; Sun, Yan; Ma, Lei; Chen, Lin; Liu, Zhengxia; Xiong, Chunrong; Yan, Guiyun; Zhu, Changliang
Resumo: The 60S ribosomal protein L22 (GenBank accession no. EF990190) was cloned from Culex pipiens pallens. An open reading frame (ORF) of 447 bps was found to encode a putative 148 amino acids protein which shares 90% and 80% identity with RPL22 genes from Aedes aegypti and Anopheles gambiae respectively. Real-time quantitative PCR analysis demonstrated that the transcription level of RPL22 in deltamethrin-resistant strain was 2.57 folds higher than in deltamethrin-susceptible strain of Cx pipiens; pallens. Overexpression of RPL22 in C6/36 cells showed that the deltamethrin-resistance was decreased in C6/36-RPL22 cell compared to the control. The mRNA level of cytochrome P450 6A1 (CYP6A1, GenBank accession no. FJ423553) showed that CYP6A1 was down-regulated in the C6/36 transfected with RPL22 (C6/36-RPL22) cells, suggesting that CYP6A1 was repressed by RPL22. Our study provides the first evidence that RPL22 may play some role in the regulation of deltamethrin-resistance in Cx pipiens pallens. (C) 2009 Published by Elsevier Inc.
Palavras-Chave: Culex pipiens pallens; Cytochrome P450 6A1; Deltamethrin resistance; Real-time quantitative PCR; Ribosomal protein L22; Transcriptional repression; Mosquito; Insect
Imprenta: Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology, v. 153, n. 2, p. 216-222, 2009
Identificador do objeto digital: 10.1016/j.cbpb.2009.03.003
Descritores: Aedes aegypti - Real Time PCR
Data de publicação: 2009
