Characterization of three mosquitocidal Bacillus thuringiensis strains isolated from urban soils of Hanoi, Vietnam
Autor(es): Yasutake, Koichi; Saitoh, Hiroyuki; Mizuki, Eiichi; Binh, Ngo Dinh; Ohba, Michio
Resumo: We characterized mosquito larvicidal activities associated with the three Vietnamese Bacillus thuringiensis soil isolates designated V-61-25 (H34, serovar konkukian), V-88-47 (H21, serovar colmeri), and V-93-38 (H34, serovar konkukian). Spore/parasporal inclusion mixtures of the three isolates induced significant larval mortalities against three species of the mosquito; Aedes aegypti, Anopheles stephensi, and Culex pipiens molestus and the moth-fly, Telmatoscopus albipunctatus. In particular, V-93-38 exhibited high mosquitocidal activities, comparable to that of the reference strain of B. thuringiensis serovar israelensis (H14). The activities of the two other isolates were 25-50 times lower than that of V-93-38, when tested on Ae. aegypti. The isolate V-93-38 differed from the israelensis strain in lacking the gene cry4A, although certain similarities were evident between the two in SDS-PAGE profiles of solubilized parasporal proteins and in Ouchterlony immunodiffusion tests.
Imprenta: Medical Entomology and Zoology, v. 58, n. 1, p. 53-58, 2007
Descritores: Aedes aegypti - Larvicide
Data de publicação: 2007
