Characterization of flagellar antigens and insecticidal activities of Bacillus thuringiensis populations in animal feces
Autor(es): Lee, DH; Shisa, N; Wasano, N; Ohgushi, A; Ohba, M
Resumo: In total, 287 Bacillus thuringiensis isolates, recovered from feces of 28 zoo-maintained animal species, were examined for flagellar (H) antigenicity and insecticidal activity. Serologically, 209 isolates (72.8%) were allocated to the 8 H serogroups, 4 were untypable, and 74 were untestable. Among the 8 H serotypes detected, H3abc (serovar kurstaki) predominated at a high frequency of 88.0%, followed by H6 (serovar entomocidus) with a frequency of 7.7%. Insecticidal activity was associated with 67.2% of the fecal populations: 188 isolates were toxic to both Bombyx mori (Lepidoptera: Bombycidae) and Aedes aegypti (Diptera: Culicidae), 2 isolates were specific for B. mori, and 3 isolates were toxic to A. aegypti only. Of the isolates with dual toxicity, 97.9% belonged to the serovar kurstaki, producing bipyramidal parasporal inclusions. All of the H7 (serovar aizawai) isolates were toxic to both insects.
Palavras-Chave: Parasporal inclusion proteins; Natural occurrence; Lectin activity; Japan; Phylloplanes; Diversity; Toxicity; Isolate; Region; Cells
Imprenta: Current Microbiology, v. 46, n. 4, p. 287-290, 2003
Identificador do objeto digital: 10.1007/s00284-002-3885-4
Descritores: Aedes aegypti - Cell ; Aedes aegypti - Immunology
Data de publicação: 2003
