Bioactivity of seagrass against the dengue fever mosquito Aedes aegypti larvae
Autor(es): Ali, M. Syed; Ravikumar, S.; Beula, J. Margaret
Resumo: To identify the larvicidal activity of the seagrass extracts. Seagrass extracts, Syringodium isotifolium Cymodocea serrulata and Halophila beccarii, were dissolved in WAS() to prepare a graded series of concentration. Batches of 21 early 4th instars larvae of Aedes aegypti (Ae. aegypti) were transferred to 250 nil, enamel bowl containing 199 mL of distilled water and I mL of plant extracts (0.01 mg - 0.1 mg). After 24 h the mortality rate was identified with the formulae [(% of test mortality - % of control morality)/100 - of control mortality] x 100. Each experiment was conducted with three replicates and a concurrent control group. A control group consisted of 1 mL of DMSO and 199 mL of distilled water only. The root extract of S. isoetifolium showed maximum larvicidal activity with minimum concentration of extract of LC50 = 0.0604 +/- 0.0040) mu g/mL with lower confidence limit (LCL) - upper confidence limit (UCL) = (0.051-0.071) and LC50 = 0.0972 mu g/mL, followed by leaf extract of S. isoetifolium showed LC50 = (0.062 +/- 0.005) mu g/mL, The regression equation of root and leaf extract of S. isoetifolium for 4th instar larvae were Y = 4.909 + 1.32x (R-2= 0.909) and Y = 2.066 + 1.21x (R-2 = 0.897) respectively. The results of the preliminary phylochemical constituents shows he presence of saponin, steroids, terpenoid, phenols, protein and sugars. From the present study the ethanolic extracts of seagrass of S. isoetifolium possesses lead compound for development of larvicidal activity
Palavras-Chave: Aedes aegypti, Bioinsecticides, Dengue fever, Larvicides, Seagrass, Syringodium isoetifolium; Cymodocea serrulata; Halophila beccarii; Mosquito; Extract
Imprenta: Asian Pacific Journal of Tropical Biomedicine, v. 2, n. 7, p. 570-573, 2012
Identificador do objeto digital: 10.1016/S2221-1691(12)60099-9
Descritores: Aedes aegypti - Larvicide ; Aedes aegypti - Public health
Data de publicação: 2012
