The effect of extrinsic incubation temperature on development of dengue serotype 2 and 4 viruses in Aedes aegypti (L.)
Autor(es): Rohani, A.; Wong, Y. C.; Zamre, I.; Lee, H. L.; Zurainee, M. N.
Resumo: Dengue 2 and 4 viruses obtained from dengue-infected patients were maintained in a C6/36 Aedes albopictus Skuse cell line and used to infect adult female Aedes aegypti mosquitoes. Each serotype was mixed separately with fresh human erythrocytes and fed to adult female mosquitoes using an artificial membrane feeding technique. Fully engorged mosquitoes were selected and retained at 26C sub(/) 28C and 30C to observe dengue virus development in Aedes vectors. Virus detection was carried out by reverse-transcriptase polymerase chain reaction (RT-PCR). The virus was first detected on Day 9 at 26C and 28C and on Day 5 at 30C for both dengue 2 and 4. The study shows the incubation period of the viruses decreased when the extrinsic incubation temperature increases.
Palavras-Chave: Temperature effects; Human diseases; Erythrocytes; Viruses; Polymerase chain reaction; Hosts; Aquatic insects; Abiotic factors; Public health; Feeding; Serotypes; Dengue; Vectors; Development; Dengue virus; Aedes aegypti; Aedes albopictus
Imprenta: Southeast Asian Journal of Tropical Medicine & Public Health, v. 40, n. 5, p. 942-950, 2009.
Descritores: Aedes aegypti - Cell ; Aedes aegypti - Infectious diseases ; Aedes aegypti - PCR detection ; Aedes aegypti - RT-PCR ; Aedes aegypti - Virus ; Aedes aegypti - Dengue ; Aedes aegypti - Public health
Data de publicação: 2009
