Lineage II of southeast Asian/American DENV-2 is associated with a severe dengue outbreak in the peruvian Amazon
Autor(es): Williams, Maya; Mayer, Sandra V; Johnson, William L; Chen, Rubing; Volkova, Evgeniya; Vilcarromero, Stalin; Widen, Steven G; Wood, Thomas G; Suarez-Ognio, Luis; Long, Kanya C; Hanley, Kathryn A; Morrison, Amy C; Vasilakis, Nikos; Halsey, Eric S
Resumo: During 2010 and 2011, the Loreto region of Peru experienced a dengue outbreak of unprecedented magnitude and severity for the region. This outbreak coincided with the reappearance of dengue virus-2 (DENV-2) in Loreto after almost 8 years. Whole-genome sequence indicated that DENV-2 from the outbreak belonged to lineage II of the southeast Asian/American genotype and was most closely related to viruses circulating in Brazil during 2007 and 2008, whereas DENV-2 previously circulating in Loreto grouped with lineage I (DENV-2 strains circulating in South America since 1990). One amino acid substitution (NS5 A811V) in the 2010 and 2011 isolates resulted from positive selection. However, the 2010 and 2011 DENV-2 did not replicate to higher titers in monocyte-derived dendritic cells and did not infect or disseminate in a higher proportion of Aedes aegypti than DENV-2 isolates previously circulating in Loreto. These results suggest that factors other than enhanced viral replication played a role in the severity of this outbreak.
Palavras-Chave: Replication; Nucleotide sequence; Viruses; Genotypes; Strains; Hygiene; Aquatic insects; Public health; Dendritic cells; Amino acid substitution; Dengue; Monocytes; Positive selection; Aedes aegypti
Imprenta: American Journal of Tropical Medicine and Hygiene, v. 91, n. 3, p. 611-620, 2014.
Descritores: Aedes aegypti - Cell ; Aedes aegypti - Virus ; Aedes aegypti - Dengue ; Aedes aegypti - Public health
Data de publicação: 2014
