Autor(es): Poupardin, Rodolphe; Srisukontarat, Wannaporn; Yunta, Cristina; Ranson, Hilary

Resumo: Temephos is the most important insecticide used in larviciding campaigns to reduce the risk of dengue transmission. This organophosphate insecticide has been in use for over 50 years and resistance to this chemical has been reported in Aedes aegypti populations from Latin America, the Caribbean and from Asia. In other insect species, organophosphate resistance is typically associated with mutations in the target site, acetylcholinesterase, that decrease the insect's sensitivity to the insecticide, or increases in the activity of one or more carboxylesterase enzymes, either by overproduction and/or amino acid substitutions, that reduce the amount of insecticide reaching the target site. Neither of these mechanisms has been previously characterised at the molecular level in dengue vectors. Here we identify an Ae aegypti carboxylesterase gene with expression levels and amino acid sequence polymorphisms correlating with temephos resistance in Thailand. This is a key step in the development of tools to manage resistance in this mosquito species.

Palavras-Chave: Population genetics; Human diseases; Insecticides; Pest control; Hosts; Biopolymorphism; Aquatic insects; Disease transmission; Public health; Amino acid substitution; Acetylcholinesterase; Dengue; Gene polymorphism; Enzymes; Carboxylesterase; Mutation; Amino acid sequence

Imprenta: Plos Neglected Tropical Diseases, v. 8, n. 3, 2014.

Descritores: Aedes aegypti - Cell ; Aedes aegypti - Transmission ; Aedes aegypti - Dengue ; Aedes aegypti - Public health

Data de publicação: 2014

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