Autor(es): Dos Santos, Joselita Maria Mendes; Fraga, Elmary C.; Maia, Juracy F.; Tadei, Wanderli P.

Resumo: Four populations of Aedes aegypti from Manaus were studied, using allozymes and RAPD loci, to determine intra- and interpopulation genetic variability and differentiation and to compare genetic structure parameters assessed with both markers. Five RAPD primers produced 52 polymorphic fragments, whereas only seven of 18 isozyme loci were polymorphic. The population from Praca 14 was the most polymorphic (P= 94.23% and P= 55.6%); while those from Coroado (P= 82.69% and P= 44.40%) and from Cidade Nova (P= 84.61% and P= 44.40%) were the least polymorphic, for both RAPD and isozymes respectively. The observed heterozygosity was higher between populations (Ho= 0.33 - 0.38) as assessed by RAPD. Wright's F statistics showed an F sub(is value higher than F) sub(s)t (F sub(is = 0.164 F) sub(s)t = 0.048). AMOVA indicated that 95.12% of the genetic variability is intrapopulational. Even so, both of the genetic markers evaluated showed a relatively high gene flow ((N sub(m= 15.15), and possibly are still random couplings, although the Fis value was not low. The genetic distance between populations was similarly low for both markers: RAPD (0.012 - 0.016) and Isozymes (0.003 - 0.016). These results show that as assessed by both markers, the populations are genetically similar, and that isozymes (codominant) are the most efficient to detect the population genetic structure. Although isozymes revealed less genetic diversity than RAPDs, the estimated levels of genetic distance were identical.)

Palavras-Chave: Population genetics; Genetic isolation; Isoenzymes; Genetic diversity; Allozymes; Population structure; Biopolymorphism; Statistical analysis; Random amplified polymorphic DNA; Heterozygosity; Differentiation; Dengue; Genetic markers; Gene flow; Primers; Genetic distance; Genetic structure; Aedes aegypti

Imprenta: Open Tropical Medicine Journal, v. 4, p. 11-20, 2011.

Descritores: Aedes aegypti - DNA ; Aedes aegypti - Dengue ; Aedes aegypti - Public health

Data de publicação: 2011

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