Detection of dengue viruses in field caught male Aedes aegypti and Aedes albopictus (Diptera: culicidae) in Singapore by type-specific PCR
Autor(es): Kow, Chung Youne; Koon, Lim Loo; Yin, Pang Fung
Resumo: Field male Aedes aegypti (L.) and Aedes albopictus (Skuse) adults caught from fixed monitoring stations weekly for 1 yr were screened for dengue viruses (DEN-1, DEN-2, DEN-3, and DEN-4). The assay was carried out using a single-step reverse transcription (or transcriptase) polymerase chain reaction (PCR) (RT-PCR) followed by a semi-nested PCR using an upstream consensus primer and four type-specific primers within the nonstructural protein three gene (NS3) of dengue viruses. The diagnostic fragments for DEN-1, DEN-2, DEN-3, and DEN-4 serotypes were of sizes 169, 362, 265, and 426 bp, respectively. Results showed that in Singapore 1.33% and 2.15% of Aedes aegypti and Aedes albopictus adult male mosquitoes, respectively, were positive for dengue viruses. The serotypes detected in male Ae. aegypti was DEN-1 (44%), followed by DEN-2 (22.2%) and DEN-3 (22.2%), and DEN-4 (11.1%). For Aedes albopictus males, the serotype was DEN-4 (38.9%), followed by DEN-2 (33.3%), DEN-3 (16.7%), and DEN-1 (11.1%).
Palavras-Chave: Diptera; Forest day mosquito; Mosquitoes; Singapore; Yellow fever mosquito; Serotypes
Imprenta: Journal of Medical Entomology, v. 38, n. 4, p. 475-479, 2001.
Descritores: Aedes aegypti - Proteins ; Aedes aegypti - Viral infections ; Aedes aegypti - PCR detection ; Aedes aegypti - RT-PCR ; Aedes aegypti - Virus ; Aedes aegypti - Dengue
Data de publicação: 2001
