Detection of dengue virus NS1 antigen in infected Aedes aegypti Using a commercially available kit
Autor(es): Voge, Natalia V.; Sanchez-Vargas, Irma; Blair, Carol D.; Eisen, Lars; Beaty, Barry J.
Resumo: Epidemic dengue has emerged throughout the tropical world. In the continued absence of a vaccine against dengue virus (DENV), mosquito vector surveillance and control programs are essential to reduce human infections. An effective test to detect DENV in infected mosquitoes would be a valuable addition to the surveillance effort. We investigated DENV detection in infected Aedes aegypti using a commercially available DENV non-structural protein 1 (NS1) ELISA kit (Platelia Dengue NS1 Ag), and by reverse transcription-polymerase chain reaction (RT-PCR) and virus isolation assays. The DENV-infected mosquitoes were subjected to field-relevant conditions and assayed individually and pooled with uninfected mosquitoes. Overall, DENV NS1 antigen was detected in 98% of infected mosquitoes/ pools versus 79% for RT-PCR and 29% for virus isolation. Our results indicate that NS1 is an excellent analyte for detection of DENV in Ae. aegypti and that the tested NS1 antigen kit provides a sensitive, rapid, and convenient test for DENV surveillance in mosquitoes.
Palavras-Chave: Biological surveys; Human diseases; Antigens; Polymerase chain reaction; Pest control; Hygiene; Aquatic insects; Public health; Enzyme-linked immunosorbent assay; Epidemics; Dengue; Control programs; Vectors; Vaccines; Infection; Dengue virus; Aedes aegypti
Imprenta: American Journal of Tropical Medicine and Hygiene, v. 88, n. 2, p. 260-266, 2013.
Descritores: Aedes aegypti - Proteins ; Aedes aegypti - Infectious diseases ; Aedes aegypti - PCR detection ; Aedes aegypti - RT-PCR ; Aedes aegypti - Virus ; Aedes aegypti - Vaccine ; Aedes aegypti - Dengue ; Aedes aegypti - Epidemic ; Aedes aegypti - Immunology ; Aedes aegypti - Public health
Data de publicação: 2013
