Autor(es): Knox, T. B.; Yen, N. T.; Nam, V. S.; Gatton, M. L.; Kay, B. H.; Ryan, P. A.

Resumo: In response to an identified paucity of information on the size and composition of immature Aedes aegypti (L.) (Diptera: Culicidae) populations in large field containers, we assessed net sampling and pumping/sieving methods for estimating and enumerating third (III)/fourth (IV) instar and pupal populations. Sweep net detection thresholds (number above which â¥90% chance of a positive sample) were â¤28 immatures for seven different container types (115-3000 liter jars and tanks) in the laboratory, and mean recovery percentages varied by container type (6.15-41.29 and 7.39-33.10% for III/IV instars and pupae, respectively). A pumping method or hand bailing was applied in the field for the collection of III/IV instars and pupae from 406 receptacles, of which 343 had been previously sampled via a five-sweep netting technique. Larvae were 9.30 times more prevalent than pupae, and abundance varied by container type with means of 36-537 III/IV instars and 6â53 pupae per receptacle. Sweep netting for III/IV instars effectively identified 86.2% of Ae. aegypti-positive containers, whereas sampling for pupae detected only 43.1% of positive containers. When conversion factors (inverse of laboratory recovery percentages) were applied to field net sampling data, estimates of container populations were more accurate for III/IV instars than pupae (maximum R super(2) = 0.610 and 0.328, respectively); however, the relationship between immature abundance and emergent adult populations remains to be defined.

Palavras-Chave: Aedes aegypti; Abundance; Net; Sampling; Surveillance

Imprenta: Journal of Medical Entomology, v. 44, n. 2, p. 192-204, 2007.

Descritores: Aedes aegypti - Public health

Data de publicação: 2007

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