Autor(es): Stevens, S. E. Jr; Murphy, R. C.; Lamoreaux, W. J.; Coons, L. B.

Resumo: Larvae of the mosquito Aedes aegypti ingested, and developed into adults, on a diet of 1O of 14 different species of cyanobacteria including Agmenellum quadruplicatum PR-6 (=Synechococcus PCC7002). Mosquito larvae ingested and grew on cells of PR-6 adapted to growth in the absence of NaCl. The cryIVD gene of Bacillus thuringiensis var. israelensis was cloned into a PR-6 expression vector to form pAQRM56, which was transformed into PR-6. Expression of the CryIVD protein in PR-6 was demonstrated by immunocytochemistry and larvicidal activity. Immunogold labelling indicated production of an electron-dense material among the thylakoid membranes of PR-6. Cells of PR-6 carrying pAQRM56 were toxic to the larvae of A. aegypti whereas control cells were not. Growth of PR-6 cells carrying pAQRM56 was slower than the growth of control cells and these cells were also larger.

Palavras-Chave: Agmenellum quadruplicatum; Synechococcus PCC 7002; Cyanobacteria; Bacillus thuringiensis var.israelensis; Mosquitocidal; Larvacide

Imprenta: Journal of Applied Phycology, v. 6, n. 2, p. 187-197, 1994.

Descritores: Aedes aegypti - Proteins ; Aedes aegypti - Cell

Data de publicação: 1994

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