Neuropeptide F and its expression in the yellow fever mosquito, Aedes aegypti
Autor(es): Stanek, DM; Pohl, J; Crim, JW; Brown, MR
Resumo: A neuropeptide F (NPF) was isolated from an extract of adult Aedes aegypti mosquitoes based on its immunoreactivity in a radioimmunoassay for Drosophila NPF. After sequencing the peptide, cDNAs encoding the NPF were identified from head and midgut. These cDNAs encode a prepropeptide containing a 36 amino acid peptide with an amidated carboxyl terminus, and its sequence shows it to be a member of the neuropeptide F/Y superfamily. Immunocytochemistry and Northern blots confirmed that both the brain and midgut of females are likely sources of NPF, found at its highest hemolymph titer before and 24 h after a blood meal. (C) 2002 Elsevier Science Inc. All rights reserved.
Palavras-Chave: Index terms; Insect; Diptera; Midgut; Peptide hormone; Neuropeptide Y
Imprenta: Peptides, v. 23, n. 8, p. 1367-1378, 2002
Identificador do objeto digital: 10.1016/S0196-9781(02)00074-8
Descritores: Aedes aegypti - DNA ; Aedes aegypti - Proteins ; Aedes aegypti - Infectious diseases
Data de publicação: 2002
