Evaluation of laboratory tests for dengue diagnosis in clinical specimens from consecutive patients with suspected dengue in Belo Horizonte, Brazil
Autor(es): Ferraz, Fernanda Oliveira; Quaresma Bomfim, Maria Rosa; Totola, Antonio Helvecio; Avila, Thiago Vinicius; Cisalpino, Daniel; Marques Pessanha, Jose Eduardo; de Souza, Danielle da Gloria; Teixeira Junior, Antonio Lucio; Nogueira, Mauriio Lacerda; Bruna-Romero, Oscar; Teixeira, Mauro Martins
Resumo: Background: Dengue is a widely spread arboviral disease in tropical and subtropical regions of the world. Dengue fever presents clinical characteristics similar to other febrile illness. Thus laboratory diagnosis is important for adequate management of the disease.Objectives: The present study was designed to evaluate the diagnostic performance of real-time PCR and serological methods for dengue in a real epidemic context.Study design: Clinical data and blood samples were collected from consecutive patients with suspected dengue who attended a primary health care unit in Belo Horizonte, Brazil. Serologic methods and real-time PCR were performed in serum samples to confirm dengue diagnosis.Results: Among the 181 consecutive patients enrolled in this study with suspected dengue, 146 were considered positive by serological criteria (positive NS1 ELISA and/or anti-dengue IgM ELISA) and 138 were positive by real-time PCR. Clinical criteria were not sufficient for distinguishing between dengue and non-dengue febrile illness. The PCR reaction was pre-optimized using samples from patients with known viral infection. It had similar sensitivity compared to NS1 ELISA (88% and 89%, respectively). We also evaluated three commercial lateral flow immunochromatographic tests for NS1 detection (BIOEASY, BIORAD and PANBIO). All three tests showed high sensitivity (94%, 91% and 81%, respectively) for dengue diagnosis.Conclusion: According to our results it can be suggested that lateral flow tests for NS1 detection are the most feasible methods for early diagnosis of dengue. (C) 2013 Elsevier B. V. All rights reserved.
Palavras-Chave: Dengue diagnosis; Real-time PCR; NS1; Anti-dengue IgM; ELISA; Lateral flow immunochromatographic assays
Imprenta: Journal of Clinical Virology, v. 58, n. 1, p. 41-46, 2013
Identificador do objeto digital: 10.1016/j.jcv.2013.06.015
Descritores: Aedes aegypti - Viral infections ; Aedes aegypti - PCR detection ; Aedes aegypti - RT-PCR ; Aedes aegypti - Serological diagnosis ; Aedes aegypti - Virus ; Aedes aegypti - Real Time PCR
Data de publicação: 2013
