An insight into the transcriptome and proteome of the salivary gland of the stable fly, Stomoxys calcitrans
Autor(es)Wang, Xuyong; Ribeiro, Jose M. C.; Broce, Alberto B.; Wilkerson, Melinda J.; Kanost, Michael R.
ResumoAdult stable flies are blood feeders, a nuisance, and mechanical vectors of veterinary diseases. To enable efficient feeding, blood sucking insects have evolved a sophisticated array of salivary compounds to disarm their host's hemostasis and inflammatory reaction. While the sialomes of several blood sucking Nematocera flies have been described, no thorough description has been made so far of any Brachycera, except for a detailed proteome analysis of a tabanid (Xu et al., 2008). In this work we provide an insight into the sialome of the muscid Stomoxys calcitrans, revealing a complex mixture of serine proteases, endonucleases, Kazal-containing peptides, anti-thrombins, antigen 5 related proteins, antimicrobial peptides, and the usual finding of mysterious secreted peptides that have no known partners, and may reflect the very fast evolution of salivary proteins due to the vertebrate host immune pressure. Supplemental Tables 51 and S2 can be downloaded from http://exon.niaid.nih.gov/transcriptome/S_calcitrans/T1/Sc-tb1-web.xls and http://exon.niaid.nih.gov/transcriptome/S_calcitrans/T2/Sc-tb2-web.xls. Published by Elsevier Ltd.
Palavras-ChaveSalivary glands; Stable fly; Hematophagy; Sialome; cDNA library; Proteome
ImprentaInsect Biochemistry and Molecular Biology, v. 39, n. 9, p. 607-614, 2009
Identificador do Objeto Digital10.1016/j.ibmb.2009.06.004
DescritoresAedes aegypti - Proteome
Data de Publicação:2009
