Amplifying whole insect genomes with multiple displacement amplification
Autor(es)Gorrochotegui-Escalante, N; Black, WC
ResumoMany applications in insect genetics require repeated analyses on individual organisms. These include population genetic and genomics, linkage mapping, molecular systematics and map-based positional cloning. However, using the polymerase chain reaction, a limited number of analyses are possible with DNA isolated from whole bodies or parts of small or preserved specimens. We describe the optimization of a new technique, Multiple Displacement Amplification, for 100-400-fold amplification of whole mosquito genomes. We demonstrate that MDA amplifies genomic DNA directly from an adult leg or from organisms as small as a first instar mosquito larva. Genetic polymorphisms revealed at individual loci are the same whether using the original genomic DNA or MDA DNA as a template.
Palavras-ChaveWhole genome amplification; Multiple displacement amplification; Genomics; Aedes aegypti; SSCP
ImprentaInsect Molecular Biology, v. 12, n. 2, p. 195-200, 2003
Identificador do Objeto Digital10.1046/j.1365-2583.2003.00401.x
DescritoresAedes aegypti - DNA ; Aedes aegypti - Genome
Data de Publicação:2003
