Genetic selection of a flavivirus-refractory strain of the yellow fever mosquito Aedes aegypti
Autor(es): Miller B.R., Mitchell C.J.
Resumo: Two inbred (isofemale) Aedes aegypti mosquito lines were derived that manifested a resistant or susceptible phenotype following ingestion of yellow fever virus; lack of virus movement from the midgut defined the resistant phenotype. Other flaviviruses, including dengue 1-4, Uganda S, and Zika, viruses behaved in a similar fashion in the two mosquito lines. Crosses between the two lines produced progeny that were of intermediate susceptibility, indicating codominance; F2 backcrosses to the parents yielded results consistent with a major controlling genetic locus and provide evidence of a second locus capable of modulating the phenotype of the major gene. The rapid selection necessary to fix the susceptible and refractory phenotypes support the hypothesis of a single major controlling locus. Viral movement across the midgut is likely to be governed by a single major gene and modifying minor genes or a group of closely linked genes. These inbred mosquito lines will be useful in discovering the molecular basis for flavivirus resistance in Ae. aegypti.
Palavras-Chave: Aedes Aegypti; Animal Experiment; Article; Dengue Virus; Female; Flavivirus; Genetic Selection; Nonhuman; Priority Journal; Virus Transmission; Yellow Fever Flavivirus; Aedes; Animal; Female; Flavivirus; Human; Inbreeding; Insect Vectors; Macaca Mulatta; Nigeria; Phenotype; Support, Non-U.S. Gov'T; Yellow Fever Virus; Aedes Aegypti; Animalia; Dengue Virus; Flavivirus; Yellow Fever Virus; Zika Virus
Imprenta: American Journal of Tropical Medicine and Hygiene, v. 45, n. 4, p. 399-407, 1991.
Descritores: Zika virus - Dengue ; Zika virus - Zika fever
Data de publicação: 1991
